INCI:Bacillus Ferment & Saccharomyces Ferment Filtrate Use Levels: 1.0% maximum Solubility:Water Soluble Appearance:Clear to Slightly Hazy Liquid
Consumer demand and ever-changing regulations has driven the market from synthetic material to focus on natural solutions. Formulator Sample Shop® prides itself in supplying effective, natural products that provide skin and hair conditioning benefits, along with providing natural antimicrobial activity. AMTicide® VAF is developed by co-fermenting Bacillus subtilis with Saccharomyces boulardii in a defined growth media to deliver a non-irritating, effective, multifunctional product. This highly marketable product can provide antioxidant benefits and is capable of preventing the growth of fungus in packaging headspace, making it the perfect addition to any formulation.
A skin moisturization study was performed using an untreated control, generic cream base, and an experimental with the same cream base containing 1.0% AMTicide® VAF. Comparative moisturization results from this study are shown in Figure 1.
As demonstrated by the results of this study, the addition of 1.0% AMTicide® VAF improved moisture levels by 16.50% after 24 hours and by 34.0% after four weeks when compared to the untreated control. When compared to the base cream AMTicide® VAF improved moisturization by 3.96% and after 24 hours and by 9.88% after four weeks. Results indicate that AMTicide® VAF is capable of increasing moisturization when compared to both the untreated control as well as the base lotion.
A Headspace Preservation Test was then conducted over a period of 28 days to evaluate the preservation adequacy of 1.0% AMTicide® VAF compared to phenoxyethanol (positive control) and an unpreserved generic cream base (negative control). Penicillium brevicompactum and Fusarium sp. were isolated from the environment via passive sedimentation, to observe a ‘real-life’ example of contamination from manufacturing areas, storage conditions, or consumer use.
The base cream formula used to perform the test was poured into individual cosmetic containers at 45°C in order to create the best environment for mold to grow under the test conditions. Each plastic jar was filled to approximately 85% of its capacity (~25ml) leaving 25% capacity of each jar empty as headspace. 1.0% AMTicide® VAF and 1.0% phenoxyethanol were subsequently added separately. Each cap of the cosmetic containers was inoculated separately with Penicillium brevicompactum and Fusarium sp. Each cap was tested at 7, 14, 21 and 28 days after the initial inoculation. Figure 2 represents the percent reduction of viable organisms after being introduced into the test formulation.
Under the conditions of this test, the AMTicide® VAF exhibited the best preservation efficacy of headspace in a cosmetic and/or personal care product container compared to phenoxyethanol. The results of this study indicate AMTicide® VAF is capable of effectively protecting products exposed to an open environment of mold contamination.
Storage: Protected from direct light and humidity at a temperature of 50°-77°F (10°-25°C)
Shelf life: 12 months, properly stored, in sealed container.
This product should be added to a formulation at the recommended usage rate